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high molecular weight poly  (InvivoGen)


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    Structured Review

    InvivoGen high molecular weight poly
    High Molecular Weight Poly, supplied by InvivoGen, used in various techniques. Bioz Stars score: 98/100, based on 4911 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+molecular+weight+poly/Poly/pmc13129108-466-25-28
    Average 98 stars, based on 4911 article reviews
    high molecular weight poly - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    High Molecular Weight:

    Article Title: Cellular and immune adaptations at the maternal-fetal interface in bats.
    Article Snippet: .. Jfb and human TOs were treated with 10 μg/mL high molecular weight poly I:C (Invivogen, tlrl-pic) or mock-treated for ∼24 h. Total RNA was extracted as described above. .. RNA-seq libraries were prepared using the Watchmaker mRNA library prep kit and sequenced on an Illumina NovaSeq X Plus platform (∼70 million 50bp paired-end reads per sample).

    Article Title: The macrophage-intrinsic MDA5/IRF5 axis drives HIV-1 intron-containing RNA-induced inflammatory responses
    Article Snippet: .. Ligands used included 3p-hpRNA (2.5 ng/mL; InvivoGen; tlrl-hprna), LPS (TLR4 ligand; 100 ng/mL; InvivoGen; tlrl-eblps), or high molecular weight poly I:C (TLR3 ligand; 10 μg/mL; InvivoGen; tlrl-pic). .. Human MDMs were derived from CD14 + monocytes isolated from PBMCs by anti-CD14 antibody–conjugated magnetic beads (Miltenyi Biotech) and cultured in RPMI supplemented with 10% Human AB Serum (Sigma-Aldrich) and 20 ng/mL M-CSF (Peprotech; 300-25) for 6 days as previously described ( , ).

    Article Title: Cellular and immune adaptations at the maternal-fetal interface in bats
    Article Snippet: .. Jfb and human TOs were treated with 10 μg/mL high molecular weight poly I:C (Invivogen, tlrl-pic) or mock-treated for ~24 h. Total RNA was extracted as described above. .. RNA-seq libraries were prepared using the Watchmaker mRNA library prep kit and sequenced on an Illumina NovaSeq X Plus platform (~70 million 50bp paired-end reads per sample).

    Article Title: Patterns and trajectories of peripheral inflammatory cytokines, immune tolerance, and lymphocyte differentiation predict transition from acute to chronic low back pain in a sex and age specific manner
    Article Snippet: .. Prior to thawing PBMCs, wells of a 96-well plate were pre-loaded with 50μl RPMI-10 containing nothing (Mock); 100ng PAM 3 CSK 4 (TLR1/2 ligand, Invivogen); 10ng lipopolysaccharide (LPS, TLR4 ligand, Invivogen); 100ng R848 (TLR7/8 ligand, Invivogen); high molecular weight poly I:C-lyovec (MDA5 ligand, Invivogen); ISD-lyovec (cGAS-STING activator, Invivogen); 500ng mouse anti-human CD40 antibody (αCD40, R&D systems MAB6321) with 2μg goat anti-mouse IgG antibody (Jackson ImmunoResearch, for crosslinking); or 200ng anti-CD3 antibody (BioLegend), 200ng Ultra-Leaf anti-CD28 antibody (BioLegend), with 1.6μg goat anti-mouse IgG (for crosslinking). .. After thawing and treating PBMCs with DNAse as described for ‘flow cytometry analyses’ above, cells were counted using a Countess II with trypan blue exclusion (Thermo-Fisher) and 5×10 4 viable PBMCs in 50μl RPMI-10 were added to wells containing each of the 8 conditions.

    Article Title: The macrophage-intrinsic MDA5/IRF5 axis drives HIV-1 intron-containing RNA-induced inflammatory responses.
    Article Snippet: .. Ligands used included 3p-hpRNA (2.5 ng/mL; InvivoGen; tlrl-hprna), LPS (TLR4 ligand; 100 ng/mL; InvivoGen; tlrl-eblps), or high molecular weight poly I:C (TLR3 ligand; 10 μg/mL; InvivoGen; tlrl-pic). .. Human MDMs were derived from CD14+ monocytes isolated from PBMCs by anti-CD14 antibody–conjugated magnetic beads (Miltenyi Biotech) and cultured in RPMI supplemented with 10% Human AB Serum (Sigma-Aldrich) and 20 ng/mL M-CSF (Peprotech; 300-25) for 6 days as previously described (29, 40).

    Article Title: Cellular and Immune Adaptations at the Maternal–Fetal Interface in Bats
    Article Snippet: .. Jfb and human TOs were treated with 10□μg/mL high molecular weight poly I:C (Invivogen, tlrl-pic) or mock-treated for ∼24 hours. ..

    Incubation:

    Article Title: SPT6 maintains epidermal homeostasis by inhibiting an NF-κB-positive feedback loop to prevent excessive inflammation
    Article Snippet: For gene knockdown experiments, passage 3 or 4 keratinocytes were transfected with 20 nM siRNA targeting SPT6 (a previously verified siRNA sequence against SUPT6H : GAGCUGAGCUGUCGAUAUA [ ]) or a nontargeting control siRNA using Lipofectamine RNAiMAX (Thermo Fisher Scientific: 13778150) according to the manufacturer’s protocol. .. The cells were incubated with the transfection reagent for approximately 18 h. After transfection for 3 days, control or SPT6 knockdown cells were incubated with high-molecular-weight poly(I: C) (InvivoGen: tlrl-pic) at 1 μg/mL for four h before harvest. .. For QNZ treatment, 5 μM QNZ (Selleck: S4902) was added to the cells for 20 h before they were harvested.

    Article Title: SPT6 maintains epidermal homeostasis by inhibiting an NF-κB-positive feedback loop to prevent excessive inflammation.
    Article Snippet: For gene knockdown experiments, passage 3 or 4 keratinocytes were transfected with 20 nM siRNA targeting SPT6 (a previously verified siRNA sequence against SUPT6H: GAGCUGAGCUGUCGAUAUA [13]) or a nontargeting control siRNA using Lipofectamine RNAiMAX (Thermo Fisher Scientific: 13778150) according to the manufacturer’s protocol. .. The cells were incubated with the transfection reagent for approximately 18 h. After transfection for 3 days, control or SPT6 knockdown cells were incubated with high-molecular-weight poly(I: C) (InvivoGen: tlrl-pic) at 1 μg/mL for four h before harvest. .. For QNZ treatment, 5 μM QNZ (Selleck: S4902) was added to the cells for 20 h before they were harvested.

    Transfection:

    Article Title: SPT6 maintains epidermal homeostasis by inhibiting an NF-κB-positive feedback loop to prevent excessive inflammation
    Article Snippet: For gene knockdown experiments, passage 3 or 4 keratinocytes were transfected with 20 nM siRNA targeting SPT6 (a previously verified siRNA sequence against SUPT6H : GAGCUGAGCUGUCGAUAUA [ ]) or a nontargeting control siRNA using Lipofectamine RNAiMAX (Thermo Fisher Scientific: 13778150) according to the manufacturer’s protocol. .. The cells were incubated with the transfection reagent for approximately 18 h. After transfection for 3 days, control or SPT6 knockdown cells were incubated with high-molecular-weight poly(I: C) (InvivoGen: tlrl-pic) at 1 μg/mL for four h before harvest. .. For QNZ treatment, 5 μM QNZ (Selleck: S4902) was added to the cells for 20 h before they were harvested.

    Article Title: SPT6 maintains epidermal homeostasis by inhibiting an NF-κB-positive feedback loop to prevent excessive inflammation.
    Article Snippet: For gene knockdown experiments, passage 3 or 4 keratinocytes were transfected with 20 nM siRNA targeting SPT6 (a previously verified siRNA sequence against SUPT6H: GAGCUGAGCUGUCGAUAUA [13]) or a nontargeting control siRNA using Lipofectamine RNAiMAX (Thermo Fisher Scientific: 13778150) according to the manufacturer’s protocol. .. The cells were incubated with the transfection reagent for approximately 18 h. After transfection for 3 days, control or SPT6 knockdown cells were incubated with high-molecular-weight poly(I: C) (InvivoGen: tlrl-pic) at 1 μg/mL for four h before harvest. .. For QNZ treatment, 5 μM QNZ (Selleck: S4902) was added to the cells for 20 h before they were harvested.

    Control:

    Article Title: SPT6 maintains epidermal homeostasis by inhibiting an NF-κB-positive feedback loop to prevent excessive inflammation
    Article Snippet: For gene knockdown experiments, passage 3 or 4 keratinocytes were transfected with 20 nM siRNA targeting SPT6 (a previously verified siRNA sequence against SUPT6H : GAGCUGAGCUGUCGAUAUA [ ]) or a nontargeting control siRNA using Lipofectamine RNAiMAX (Thermo Fisher Scientific: 13778150) according to the manufacturer’s protocol. .. The cells were incubated with the transfection reagent for approximately 18 h. After transfection for 3 days, control or SPT6 knockdown cells were incubated with high-molecular-weight poly(I: C) (InvivoGen: tlrl-pic) at 1 μg/mL for four h before harvest. .. For QNZ treatment, 5 μM QNZ (Selleck: S4902) was added to the cells for 20 h before they were harvested.

    Article Title: SPT6 maintains epidermal homeostasis by inhibiting an NF-κB-positive feedback loop to prevent excessive inflammation.
    Article Snippet: For gene knockdown experiments, passage 3 or 4 keratinocytes were transfected with 20 nM siRNA targeting SPT6 (a previously verified siRNA sequence against SUPT6H: GAGCUGAGCUGUCGAUAUA [13]) or a nontargeting control siRNA using Lipofectamine RNAiMAX (Thermo Fisher Scientific: 13778150) according to the manufacturer’s protocol. .. The cells were incubated with the transfection reagent for approximately 18 h. After transfection for 3 days, control or SPT6 knockdown cells were incubated with high-molecular-weight poly(I: C) (InvivoGen: tlrl-pic) at 1 μg/mL for four h before harvest. .. For QNZ treatment, 5 μM QNZ (Selleck: S4902) was added to the cells for 20 h before they were harvested.

    Knockdown:

    Article Title: SPT6 maintains epidermal homeostasis by inhibiting an NF-κB-positive feedback loop to prevent excessive inflammation
    Article Snippet: For gene knockdown experiments, passage 3 or 4 keratinocytes were transfected with 20 nM siRNA targeting SPT6 (a previously verified siRNA sequence against SUPT6H : GAGCUGAGCUGUCGAUAUA [ ]) or a nontargeting control siRNA using Lipofectamine RNAiMAX (Thermo Fisher Scientific: 13778150) according to the manufacturer’s protocol. .. The cells were incubated with the transfection reagent for approximately 18 h. After transfection for 3 days, control or SPT6 knockdown cells were incubated with high-molecular-weight poly(I: C) (InvivoGen: tlrl-pic) at 1 μg/mL for four h before harvest. .. For QNZ treatment, 5 μM QNZ (Selleck: S4902) was added to the cells for 20 h before they were harvested.

    Article Title: SPT6 maintains epidermal homeostasis by inhibiting an NF-κB-positive feedback loop to prevent excessive inflammation.
    Article Snippet: For gene knockdown experiments, passage 3 or 4 keratinocytes were transfected with 20 nM siRNA targeting SPT6 (a previously verified siRNA sequence against SUPT6H: GAGCUGAGCUGUCGAUAUA [13]) or a nontargeting control siRNA using Lipofectamine RNAiMAX (Thermo Fisher Scientific: 13778150) according to the manufacturer’s protocol. .. The cells were incubated with the transfection reagent for approximately 18 h. After transfection for 3 days, control or SPT6 knockdown cells were incubated with high-molecular-weight poly(I: C) (InvivoGen: tlrl-pic) at 1 μg/mL for four h before harvest. .. For QNZ treatment, 5 μM QNZ (Selleck: S4902) was added to the cells for 20 h before they were harvested.



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